Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
نویسندگان
چکیده
منابع مشابه
Small-angle scattering studies of biological macromolecules in solution
Small-angle scattering (SAS) of x-rays and neutrons is a fundamental tool in the study of biological macromolecules. The major advantage of the method lies in its ability to provide structural information about partially or completely disordered systems. SAS allows one to study the structure of native particles in near physiological environments and to analyse structural changes in response to ...
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be 35.1 kJ/mol (8.4 kcal/mol) for formation of the complex and 115.9 kJ/mol(27.7 kcal/mol) for dissociation of the complex. The kinetic results, as well as helping to describe and validate the model of hyaluronate-proteoglycan interaction, are also of value in the design of experiments. In the experiment shown in Fig. 1 equilibrium is only attained after approx. 1-lfh, as monitored by light-sca...
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Synchrotron small angle x-ray scattering (SAXS) has been applied to the structural study of several biological systems, including the nitrogenase complex, the heat shock cognate protein (hsc70), and lysozyme folding. The structural information revealed from the SAXS experiments is complementary to information obtained by other physical and biochemical methods, and adds to our knowledge and unde...
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Small-angle scattering of X-rays (SAXS) is an established method for the low-resolution structural characterization of biological macromolecules in solution. The technique provides three-dimensional low-resolution structures, using ab initio and rigid body modeling, and allow one to assess the oligomeric state of proteins and protein complexes. In addition, SAXS is a powerful tool for structure...
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ABSTRACT Background: Coherent scattering leads to diffraction effects and especially constructive interferences. Theseinterferences carry some information about the molecular structure of the tissue. As breast cancer isthe most widespread cancer in women, this project evaluated the application of small angleX-ray scattering (SAXS) for differentiation between normal and cancerous breast tissues....
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2018
ISSN: 1940-087X
DOI: 10.3791/58538